Absolutely NOT!! We love our fur baby and are cruelty-free, so we do NOT test on animals. Related articles ➤ Will the Like A Virgin Hair Masque repair split ends? ➤ Does the hair masque contain sulfates? ➤ What is the Tangle Tamer made of? ➤ Is the hair masque 100% vegan? ➤ Can you send me a sample before purchasing the hair masque?